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Literature summary extracted from

  • Vincenzetti, S.; Cambi, A.; Neuhard, J.; Garattini, E.; Vita, A.
    Recombinant human cytidine deaminase: Expression, purification, and characterization (1996), Protein Expr. Purif., 8, 247-253.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.5 cDNA, PCR, vector pTrc99A, cloned and expressed in Escherichia coli DH5alpha Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.5
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.5 3,4,5,6-Tetrahydrouridine recombinant CDA Homo sapiens
3.5.4.5 3-deazauridine recombinant enzyme Homo sapiens
3.5.4.5 5-fluoro-2'-deoxyuridine recombinant enzyme Homo sapiens
3.5.4.5 5-{(1E)-3-[(5-carboxypentyl)amino]-3-oxoprop-1-en-1-yl}-5,6-dihydrocytidine recombinant enzyme Homo sapiens
3.5.4.5 CMP recombinant CDA Homo sapiens
3.5.4.5 thymine riboside recombinant CDA Homo sapiens
3.5.4.5 uridine
-
 Escherichia coli
3.5.4.5 uridine recombinant enzyme Homo sapiens
3.5.4.5 UTP recombinant CDA Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.5 0.039
-
 cytidine recombinant CDA Homo sapiens
3.5.4.5 0.039
-
 deoxycytidine recombinant CDA Homo sapiens
3.5.4.5 0.04
-
 5-methylcytidine recombinant CDA Homo sapiens
3.5.4.5 0.1
-
 5-iododeoxycytidine recombinant CDA Homo sapiens
3.5.4.5 0.11
-
 cytosine arabinoside recombinant CDA Homo sapiens
3.5.4.5 0.28
-
 5-azacytidine recombinant CDA Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.5 15000
-
 4 * 15000, SDS-PAGE, purified enzyme, homotetramer Homo sapiens
3.5.4.5 16000
-
 estimated from cDNA open reading frame coding for human cytidine deaminase Homo sapiens
3.5.4.5 16170
-
 deduced from cDNA nucleotide sequence Homo sapiens
3.5.4.5 32000
-
 2 * 32000, homodimer, SDS-PAGE Escherichia coli
3.5.4.5 57000
-
 gel filtration Escherichia coli
3.5.4.5 57000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.5 cytidine + H2O Bacillus subtilis
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Escherichia coli
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Homo sapiens
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Escherichia coli DH5-alpha
-
 uridine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Bacillus subtilis
-
-
-
3.5.4.5 Escherichia coli
-
 strain DH5alpha
-
3.5.4.5 Escherichia coli DH5-alpha
-
 strain DH5alpha
-
3.5.4.5 Homo sapiens
-
 human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.5
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.5 granulocyte
-
 Homo sapiens
-
3.5.4.5 leukocyte
-
 Homo sapiens
-
3.5.4.5 liver
-
 Homo sapiens
-
3.5.4.5 lymphocyte
-
 Homo sapiens
-
3.5.4.5 placenta
-
 Homo sapiens
-
3.5.4.5 spleen
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.5 105
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.5.4.5 -20°C, 20 mM Tris-HCl, pH 7.5, 1 mM DDT, 1 mM EDTA, 20% glycerol, enzyme retains full activity for at least 1 month, 80% of activity lost in absence of glycerol Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 1-beta-D-arabinofuranosylcytosine + H2O
-
 Escherichia coli ?
-
 ?
3.5.4.5 1-beta-D-arabinofuranosylcytosine + H2O
-
 Homo sapiens ?
-
 ?
3.5.4.5 1-beta-D-arabinofuranosylcytosine + H2O
-
 Escherichia coli DH5-alpha ?
-
 ?
3.5.4.5 5-azacytidine + H2O
-
 Homo sapiens 5-azauridine + NH3
-
 ?
3.5.4.5 5-azadeoxycytidine + H2O
-
 Homo sapiens 5-azadeoxyuridine + NH3
-
 ?
3.5.4.5 5-methylcytidine + H2O
-
 Homo sapiens 5-methyluridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Bacillus subtilis uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Escherichia coli uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Homo sapiens uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Escherichia coli DH5-alpha uridine + NH3
-
 ?
3.5.4.5 deoxycytidine + H2O
-
 Escherichia coli deoxyuridine + NH3
-
 ?
3.5.4.5 deoxycytidine + H2O
-
 Homo sapiens deoxyuridine + NH3
-
 ?
3.5.4.5 deoxycytidine + H2O
-
 Escherichia coli DH5-alpha deoxyuridine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.5 dimer 2 * 32000, homodimer, SDS-PAGE Escherichia coli
3.5.4.5 tetramer
-
 Bacillus subtilis
3.5.4.5 tetramer 4 * 16000, amino acid sequence Homo sapiens
3.5.4.5 tetramer 4 * 15000, SDS-PAGE, purified enzyme, homotetramer Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.5 3.5 10.5 deoxycytidine as substrate, beyond these values irreversible inactivation occurs Homo sapiens